Table 1.
Combinatorial mutagenesis datasets analyzed in this study
| Protein | Genotype space | Phenotypes |
|---|---|---|
| Methyl-parathion hydrolase49 | 25 (32) | Catalytic activity |
| β-lactamase51 | 25 (32) | Antibiotics resistance (minimum antibiotics conc. inhibiting growth) |
| Dihydrofolate reductase3 | 3 × 24 (48) | Antibiotics resistance (antibiotics conc. reducing growth rate by 75%) |
| Influenza A H3N2 hemagglutinin41 | 22 × 32 × 42 (576) | Viral replication fitness |
| Antibody CR626119 | 211 (2,048) | Affinity for influenza hemagglutinin strain H1 or H9 |
| Bacterial antitoxin ParD352 | 203 (8000) | Fitness conferred by binding to toxin ParE3 or ParE2 |
| Aequorea victoria GFP (avGFP)15 | 213 (8192) | Fluorescence |
| Bacterial antitoxin ParD353 | 13 × 12 × 10 × 6 (9360) | Fitness conferred by binding to toxin ParE3 or ParE2 |
| SARS-CoV-2 spike protein7 | 215 (32,768) | Affinity for human ACE2 |
| Antibody CH6520 | 216 (65,536) | Affinity for influenza hemagglutinin strain MA90, MA90-G189E, or SI06 |
| Antibody CR911419 | 216 (65,536) | Affinity for influenza hemagglutinin strain B, H1, or H3 |
| Transcription factor ParB50 | 204 (160,000) | Fitness conferred by transcription |
| Protein G B1 domain (GB1)12 | 204 (160,000) | Binding enrichment for IgG-Fc |