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. 2024 Sep 11;15:7957. doi: 10.1038/s41467-024-51938-x

Fig. 2. IL-1R1 signaling modulates body weight, WAT mass, adipocyte size, and new fat cell formation.

Fig. 2

a–d Body weight development (a), body weight (b), fat pad mass (c, d) of 17-week-old chow and HFD-fed male mice. n = 12 WT-CD, n = 12 IL1R1-KO-CD, n = 14 WT-HFD, n = 14 IL1R1-KO-HFD. e–h Adipocyte size distribution (e–h) of 17-week-old chow and HFD-fed male mice. n = 10 IL1R1-WT-CD, n = 12 IL1R1-KO-CD (e). n = 14 per genotype (f). n = 12 IL1R1-WT-CD, n = 10 IL1R1-KO-CD (g). n = 14 IL1R1-WT-HFD, n = 13 IL1R1-KO-HFD (h). i–n Body weight development (i), body weight (j), fat pad mass (k, l) and adipocyte size distribution (m, n) of 17-week-old HFD-fed male mice treated with IL-1Ra (10 mg/kg bw daily for 14 days, n = 16 for both conditions). o, p Relative Il1r1 mRNA expression in adipocytes, CD45–, and CD45+ stromal vascular cells isolated from gWAT (o) and scWAT (p) of 12-week-old chow-fed male WT mice (n = 5). q–s Experimental scheme of EdU tracing experiments (100 μg EdU/day, every 2 days, total of 4 injections) (q) and percentage of EdU+ adipocyte nuclei isolated from gWAT (r) and scWAT (s) of IL1R1-KO mice and WT controls. gWAT IL1R1-KO and gWAT WT: chow diet (n = 12 per genotype); HFD (n = 14 per genotype). scWAT IL1R1-KO and scWAT WT: chow diet (n = 11 per genotype); HFD (n = 12 per genotype). t–v Experimental scheme of EdU tracing experiments (100 μg EdU/day, every 2 days, total 4 injections) and IL-1Ra therapy (10 mg/kg bw daily for 14 days) (t) and percentage of EdU+ adipocyte nuclei isolated from gWAT (u) and scWAT (v) of HFD-fed WT mice. gWAT: saline and IL-1Ra (n = 16). scWAT: saline (n = 16); IL-1Ra (n = 13). Scale bar = 200 µm. Experimental schemes created with biorender.com. n = biological replicates. Data are shown as individual measurements and mean ± SEM. Statistical analyses were performed by: unpaired nonparametric two-tailed Mann-Whitney U test (j–l, u, v); one-way ANOVA and Šidák’s multiple comparison test (o, p); or two-way ANOVA and Šidák’s (a, e–i, m, n) or Fisher’s LSD (b–d, r, s) multiple comparison test. Source data are provided as a Source Data File.