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. 2024 Sep 11;15:7937. doi: 10.1038/s41467-024-52325-2

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© The Author(s) 2024

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 2 — a Representative chromatograms of purified V. cholerae Δldt mutant sacculi treated with LdtA for varying times. The percentage of LD-crosslinking is indicated on the right. b Activity of V. cholerae LTs (MltA, MltB, MltD, MltG and Slt70) on substrates with different LD-crosslinking levels relative to the sacculi substrate with 0% LD-crosslinks. c Relative activity of V. cholerae LTs (MltA, MltB, MltD, MltG and Slt70) on V. cholerae wild-type (Vc WT) or Δldt mutant (Vc Δldt) sacculi. Activity is calculated relative to the Δldt sacculi substrate, with 0% LD-crosslinks. d Relative activity of V. cholerae LTs (MltA, MltB, MltD, MltG and Slt70) on Photobacterium damselae wild-type (Pd WT) or Δldt mutant (Pd Δldt) sacculi. Activity is calculated relative to the Δldt sacculi substrate, with 0% LD-crosslinks. All in vitro assays were performed in triplicate. Data are presented as mean values +/- standard deviation. Source data are provided as a Source Data file.