FIG. 8.

CW3 binds a discontinuous epitope within the first CRP of HveA. CW1 and CW3 were tested for reactivity with HveA(76t) and HveA(77–120t) by ELISA. Twofold dilutions of CW1 and CW3 were added to the wells of ELISA plates coated with HveA(76t) or HveA(77–120t). Bound MAb was detected with horseradish peroxidase-conjugated anti-mouse IgG and horseradish peroxidase substrate. Plates were read at 405 nm, and the absorbance in each well was plotted against the MAb concentration. □, CW1; ○, CW3. (A) ELISA plate coated with HveA(76t). (B) ELISA plate coated with HveA(77–120t). (C) Western blot testing of CW1 and CW3 for reactivity with HveA(200t) or reduced and alkylated HveA(200t). For each blot, lane 1 contained HveA(200t) and lane 2 contained reduced and alkylated HveA(200t). Blots were probed with the indicated MAbs. The position of the band corresponding to HveA(200t) is indicated by an arrow.