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. 2024 Sep 11;7(11):e202402918. doi: 10.26508/lsa.202402918

Figure 2. Improved mitochondrial reporter tools in C. elegans.

Figure 2.

Comparative analysis of mitochondrial visualization techniques in various tissues C. elegans on day 2 of adulthood. The upper panel of images demonstrates the variable staining efficacy of TMRE in WT N2 worms. The novel SKI LODGE strains, expressing the TOMM-20 transmembrane domain fragment fused to GFP or mCherry, respectively, under the eft-3p promoter, exhibit more uniform labeling. Endogenous mitochondrial strains include, GFP expression on the translocase TOMM-70, or mScarlet tagging on the translocase of the inner mitochondrial membrane TIMM-50, respectively. Dual labeling of both mitochondrial membranes is achieved in a strain expressing GFP on the OMM and mScarlet on the IMM. Scale bar (head, germline): 20 μm. Scale bar (muscle, intestine, hypodermis): 10 μm.