Figure S3. Effect of antibiotics on the SKI LODGE TOMM-20^aa1–49::GFP C. elegans strain.

Mitochondria in muscle cells of (SKI LODGE) TOMM-20^aa1–49::GFP animals exposed to a combination of the antibiotics kanamycin (0.05 mg/ml) and carbenicillin (0.1 mg/ml) for 24 h were more fragmented compared with control animals that were not exposed to antibiotics. Representative images from 20 to 30 animals. Quantification of mitochondrial fragmentation is represented by mitochondrial length and circularity measured by MitoMAPR. *P < 0.05, ***P < 0.001 by two-tailed t test. Scale bar: 10 μm.