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Comparison of young (Day 1) and old (Day 8) worms revealed the development of protein aggregates associated with aging, consistently observed in strains utilizing red fluorophores, including mScarlet, RFP, or mCherry. This phenomenon was exclusive to red fluorophore tags, as TOMM-20aa1–49::GFP or TOMM-70::GFP animals, respectively, did not exhibit protein aggregation. Scale bar: 5 μm.
