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. Author manuscript; available in PMC: 2025 Apr 1.
Published in final edited form as: Nature. 2024 Apr 17;629(8010):121–126. doi: 10.1038/s41586-024-07297-0

Figure 2: Expression of catecholamine biosynthetic enzymes by lamprey sympathoblasts.

Figure 2:

(a-j) HCR detection of Th (teal) and Dbh (red) in lamprey at T28. Co-localization of the transcripts is seen in cells surrounding the heart (b-d) and spanning the initial length of the trunk in bilateral streams (e-j). Scale bars=100μm. n=15 embryos across five independent replicates. (k-m) Immunohistochemical detection of TH (teal) at T28 is consistent with HCR. HNK1 antibody staining of axons is shown in red. Scale bars=100μm. n=15 embryos across five independent replicates. (n, o) Immunohistochemical detection of TH (red) at (n) T27 and (o) ammocete stages. At both stages, TH+ cells are localized lateral to the dorsal aorta (denoted by white asterisks) and dorsal to the yolk tube or the intestinal epithelium. TH+ cells are highlighted in zoomed insets. DAPI is shown in white. Scale bars=50μm. n=12 embryos, 12 ammocetes across four independent replicates.