TABLE 1.
Plasmids used in this study
| Plasmid | Description | Source |
|---|---|---|
| pAS2-1 | Gal4 DNA-binding domain in a 2μm TRP1 yeast shuttle vector | Clontech Inc. |
| pACT2 | Gal4 activation binding domain in 2μm LEU2 yeast shuttle vector | Clontech Inc. |
| pFG105 | NS3 (aa 1–680) in pAS2-1 | This work |
| pFG106 | NS3 (aa 1–680) in pACT2 | This work |
| pFG109 | myc-NS3 in pXJ40 | This work |
| pFG114 | Flag-NS3 in pXJ40 | This work |
| pFG134 | pAS-CΔ-Stul (NS3 aa 1–538) | This work |
| pFG135 | pAS-CΔ-AflIII (NS3 aa 1–430) | This work |
| pFG136 | pAS-CΔ-BstEII (NS3 aa 1–328) | This work |
| pFG137 | pAS-CΔ-SspI (NS3 aa 1–335) | This work |
| pFG138 | pAS-CΔ-SphI (NS3 aa 1–149) | This work |
| pFG146 | pAS-NΔ-Smal (NS3 aa 162–680) | This work |
| pFG147 | pAS-NΔ-NdeI (NS3 aa 247–680) | This work |
| pFG148 | pAS-NΔ-Sspl (NS3 aa 336–680) | This work |
| pFG150 | pAS-min (SmaI-SspI, NS3 aa 162–335) | This work |
| pFG156 | pAS-NΔ-Ncol (NS3 aa 57–680) | This work |
| pFG157 | pAS-helicase (NS3 aa 182–680) | This work |
| pFG158 | pACT-helicase (NS3 aa 182–680) | This work |
| pFG159 | pAS-Smal-Ndel (NS3 aa 162–246) | This work |
| pFG161 | pAS-Ndel-Sspl (NS3 aa 247–335) | This work |
| pFG162 | pGEX-helicase (NS3 aa 182–680) | This work |
| pFG166 | pFG150 cut with EagI and BstEII and religated | This work |
| pFG171 | pACT-min (SmaI-SacI, NS3 aa 182–375) | This work |
| pFG188 | myc-min (NS3 aa 182–375) in pXJ40 | This work |
| pXJ40 | Mammalian expression vector with cytomegalovirus promoter, Kozak sequence, and simianvirus 40 poly(A) termination signal | 28 |
| pXJ40-myc | Mammalian expression vector for tagging proteins with c-myc at the N terminus | V. Yu lab, IMCBa |
| pXJ40-flag | Mammalian expression vector for tagging proteins with Flag at the N terminus | 21 |
| pGEX-2TX | GST fusion expression vector with modified multiple cloning sites | S. C. Lin lab, IMCBa |
a
IMCB, Institute of Molecular and Cell Biology, Singapore.