Skip to main content
. 2024 Aug 23;9(36):37748–37753. doi: 10.1021/acsomega.4c03216

Figure 2.

Figure 2

Effect of the number of azobenzenes on the dissociation of the MT swarm. a. Fluorescence microscopy images showing the dissociation of the MT swarms upon varying the number of azobenzene, Z (n) in T12(TTG)(n+1)Z(n) DNA after 5 min of UV irradiation. The intensity of UV light is 0.6 mW/cm2. The association images were captured after 60 min of VIS light irradiation. The labeling ratio of DNA to MT was maintained the same. Scale bar: 20 μm. b. Change in the association ratio of the MTs with the UV irradiation time in which MTs were modified with different numbers of azobenzene-incorporated DNA. Error bar: standard deviation. The inset shows the enlarged association ratio from 0 to 10 min. c. Change in the relaxation time (τ) of MT dissociation from the swarm under UV irradiation with the number of azobenzenes, Z (n) in DNA. Relaxation time was measured from the fitted curve of Figure 2b; the data were fitted with Y = (Y0 - Plateau)*exp(-K*X) + Plateau, where K is the rate constant). Relaxation time is the characteristic time for dissociation, which is computed as the reciprocal of the rate constant. The best fit line has a slope of −0.31 ± 0.08 and a goodness of fit of R2 = 0.82. (Y0 – Plateau), the amplitude of the exponential decay is found to increase with the Azobenzene number of DNA.