Fig. 5.

Capsaicin reduced MLE12 cell injury and ferroptosis after cyclic stretching. A Cell viability of CS-MLE12 cells after treatment with different doses of CAP (n = 5). B, C Immunofluorescence photomicrographs and quantitative analysis of PGSK (n = 3, Scale bar: 100 μm). D, E Immunofluorescence photomicrographs and quantitative analysis of C11-BODIPY.^581/591 in MLE12 cells (n = 3, Scale bar: 50 μm). F Representative western blots of SLC7A11, GPX4, and β-actin in MLE12 cells. G Relative protein expression of SLC7A11 and GPX4 was normalized to β-actin (n = 3). Data are expressed as mean ± standard deviation. “*” indicates a significant difference between the corresponding groups (*p < 0.05, **p < 0.01, or ***p < 0.001)