Fig. 7.

Knockdown SIRT3 attenuates the beneficial effect of capsaicin on mitochondrial oxidative damage and ferroptosis in vitro. A Levels of ATP (n = 3). B Levels of glutathione (GSH) (n = 3). C Levels of Mn-SOD (n = 3). D Levels of MDA (n = 3). E Representative Western blots of SIRT3, SLC7A11, GPX4, and β-actin in MLE12 cells. F–H Relative protein expression of SIRT3, SLC7A11, and GPX4 normalized to β-actin (n = 3). Data are expressed as mean ± standard deviation. “*” indicates a significant difference between the corresponding groups (*p < 0.05, **p < 0.01, or ***p < 0.001)