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. 2024 Sep 5;13(9):bio060542. doi: 10.1242/bio.060542

Fig. 5.

Fig. 5.

HMGB1 content is increased in one model of mdx mice and restored to WT levels with microdystrophin treatment. (A) HMGB1 fluorescence intensity in nuclei and myofiber membranes appears to be increased in B10- and D2-mdx+VEH muscle as compared to B10-WT+VEH, D2-WT+VEH, B10-mdx+AAV, and D2-mdx+AAV samples. (B) There appears to be an increase in VCAM1 nuclear expression in B10-mdx+VEH and D2-mdx+VEH muscle. (C/E) HMGB1 protein content was significantly increased in B10-mdx+VEH muscle isolates (P-value <0.0001) and decreased with microdystrophin treatment (P-value=0.0011). (D/F) There was no change in HMGB1 protein content between D2 groups. (G) B10-mdx+VEH mice showed a significant increase in Vcam1 but not Hmgb1 transcript expression as compared to B10-WT+VEH mice. (H) There were no significant differences in Hmgb1 or Vcam1 transcript expression across D2 groups. (NTC, no tissue control). P-value **≤0.01, ***≤0.001.