Table 1.

AssayMAP Bravo software parameters for the four deck layouts shown in Supplemental Table 1.

Antibody binding
Step	Volume	Flow Rate	Buffer
Prime	100 μL	300 μL/min	1X PBS
Equilibrate	50 μL	10 μl/min	1X PBS
Load Sample	125 μL	3 μL/min	Antibody in PBS
Cup Wash 1	25 μL		1X TBS
Internal Cartridge Wash 1	50 μL	10 μL/min	1X TBS
Pre-clearing cell lysate
Step	Volume	Flow Rate	Buffer
Prime	100 μL	300 μL/min	1X PBS
Equilibrate	50 μL	10 μL/min	1X PBS
Load Sample	800 μL	45 μL/min	Cell Lysate
Immunoprecipitation
Step	Volume	Flow Rate	Buffer
Prime	100 μL	300 μL/min	1X PBS
Equilibrate	50 μL	10 μL/min	1X PBS
Load Sample	800 μL	10 μL/min	Pre-cleared lysate
Cup Wash 1	25 μL		1x TBS
Internal Cartridge Wash 1	100 μL	10 μL/min	1x TBS
Stringent Syringe Wash	50 μL		10% Acetic Acid
Elute	100 μL	5 μL/min	10% Acetic Acid
Peptide desalting
Step	Volume	Flow Rate	Buffer
Prime	100 μL	300 μL/min	50% ACN, 0.1% FA
Equilibrate	50 μL	10 μL/min	0.1% FA
Load Sample	100 μL	5 μL/min	Previous eluate
Cup Wash	25 μL		0.1% FA
Internal Cartridge Wash	50 μL	10 μL/min	0.1% FA
Stringent Syringe Wash	50 μL		50% ACN, 0.1% FA
Elute	40 μL	5 μL/min	50% ACN, 0.1% FA