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. Author manuscript; available in PMC: 2024 Sep 12.
Published in final edited form as: J Proteome Res. 2020 Dec 17;20(1):393–408. doi: 10.1021/acs.jproteome.0c00464

Table 3.

Sequential pMHC II elutions using RP-S cartridges

Elution1 PSMs Unique peptides
Rep1 1 14760 8768
2 1903 984
3 81 65
4 7 7
5 8 2
Total 16759 8968
Rep2 1 14401 8650
2 2713 1477
3 517 279
4 116 116
5 29 21
Total 17776 8902
1.

Five sequential 20 μL elution steps were performed on RP-S cartridges using pMHC II immunoprecipitated from 1×108 Raji Cells (N=2) (see Supplemental Table 1 for details).