Table 1. Shortened list of potential suppressor mutations: The mutations that could potentially suppress the sporulation defect in EMS treated isolates.

The underlined strains were derived from the C. difficile ΔsspB* strain while the nonunderlined strains were derived from the C. difficile ΔsspA ΔsspB strain.

Gene	Function	Isolates	Mutation
rpoB	RNA polymerase beta subunit	HNN37	P893S
rpoC	RNA polymerase beta’ subunit	HNN40	H94Y
rpoC	RNA polymerase beta’ subunit	HNN32	W225 stp
rpoC	RNA polymerase beta’ subunit	HNN40, HNN22	S315F
rpoA	RNA polymerase alpha subunit	HNN51, HNN19	S281F
CDR20291_0714	Stage IV sporulation protein	HNN19	A20T
CDR20291_0714	Stage IV sporulation protein	HNN33, HNN35, HNN37, HNN38, HNN41, HNN48	F37F; Synonymous
sigG	Forespore sporulation sigma factor	HNN39	M97I
sspA	Small acid soluble protein	HNN26, HNN28	V52G; Reversion
spoVT	Stage V sporulation protein	HNN51	P39S