FIG. 1.

SDS-PAGE analysis of metabolically labeled proteins from vaccinia virus-infected cells. BS-C-1 cells were uninfected (U) or were infected with vaccinia virus WR (W) or recombinant vaccinia viruses from which the A33R ORF (ΔA33) or the A36R ORF (ΔA36) had been deleted. After 8 h, infected and uninfected cells were labeled with H₃³²PO₄ (A, C, and D) or [³⁵S]methionine (B, C, and D) for 18 h. Lysates were immunoprecipitated with either α-A33R (α-A33) or α-A36RC (α-A36). In panel D, cells were infected in the absence (−) or presence (+) of rifampin (Rif). Immunoprecipitated proteins were run on SDS-PAGE gels under reducing conditions (A, B, and D) or nonreducing conditions (C) and visualized by autoradiography. Masses of marker proteins (in kilodaltons) are given on the left. Arrows point to the 28-kDa A33R or the 50-kDa A36R protein.