FIG. 10.

ORF10p immunoreactivity is found in the cytosol, in coatings of Golgi-TGN membranes, and in virions in cells infected with VZV. (A) Intact (Ellen) VZV. ORF10p immunoreactivity is demonstrated with 10-nm particles of immunogold in the region of the Golgi apparatus. Gold particles are found in the cytosol and in the dense material coating membranes of the Golgi stack (arrows). (B) Late endosomes-prelysosomes in a cell infected with intact (Ellen) VZV. ORF10p immunoreactivity is found within pleomorphic virions (arrow) that accumulate within these vacuoles. (C) gI_ΔN mutant virus. ORF10p immunoreactivity is found in the osmiophilic material (arrows) that coats the membranes of the rings and stacks of adherent cisternae that characterize the TGN of cells infected with gI mutant virions (see Fig. 5). (D) gI_ΔN mutant virus. ORF10p immunoreactivity is found in the virions within the chambers (arrows) and the coating of the membranes that form the walls of the chamber of the honeycomb that characterizes the TGN of cells heavily infected with gI mutant virions. Bars, 150 nm.