Fig. 2.

Antiproliferative effect of domperidone on HCT116 cells. (A) HCT116 cells were treated with domperidone (0, 5, 10, 25, 50, and 100 μM) for 24, 48, and 72 h, and cell viability was determined using the MTS cell viability assay. Data are shown as the mean ± standard deviation (SD), (n=3). (B) Representative images of domperidone-treated HCT116 cells. The scale bar indicates 200 μm. (C, D) HCT116 cells treated with varying domperidone concentrations (0, 5, 10, 25, and 50 μM) for 48 h were analyzed by FACS with Annexin V/PI staining. Data are shown as the mean ± SD, (n=3). **p<0.01.