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. 2023 Nov 16;42(9):1404–1416. doi: 10.1038/s41587-023-01977-4

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Extended Data Fig. 4 — a, qPCR of gene expression following NE neuronal differentiation at day 11, 19, 30 and 40. Data are shown as mean ± s.d. n = 3 biologically independent samples for each condition. b, c, Immunostaining for HCRT1 and OPRM1 in H9 derived NE neurons at day 30. Scale bars, 20 μm. d, The expression of CRHR1 by western blot at Day 0 (ES), Day 18 (D18) and Day 30 (D30) along NE differentiation. e, f, Immunostaining for NPY and GAL in H9 derived NE neurons at day 30. scale bars, 50 μm. g, Immunostaining for GAL and NET in mouse LC to demonstrate the specificity of the GAL antibody. Scale bar, 50 μm. h,Immunostaining for PRPH (Peripherin) in hPSC derived LC-NE neurons at day 30. A positive control to demonstrate the specificity of the peripherin antibody is provided using hPSC derived neurons. Scale bars, 20 μm.

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