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. 2024 Jan 2;42(9):1378–1383. doi: 10.1038/s41587-023-01983-6

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© The Author(s) 2024

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Extended Data Fig. 1 — In order to estimate the degree of fragmentation of assemblers, we aligned the contigs of one assembler against the near-complete circular contigs (cMAGs) recovered by the other assemblers. The fragmentation is then represented as the number of contigs required to cover these cMAGs (see section ‘Assessment of completeness and fragmentation of assemblies using reference sequences’ for details). The boxplot elements are the median (horizontal bar), 25th and 75th percentiles (box limits Q1 and Q3), Q1-1.5*IQR and Q3+1.5*IQR (whiskers, IQR=Q3-Q1) and outliers. Summary statistics (n, min, median, mean, max): Human gut- metaMDBG (19, 1, 2, 2.1, 5); hifiasm-meta (32, 1, 2, 4, 24); metaFlye (68, 1, 4, 7.5, 48) : AD-HiFi- metaMDBG (11, 1, 2, 2.3, 6); hifiasm-meta (72, 1, 6, 19.8, 109); metaFlye (105, 1, 6, 15.1, 104) : Sheep rumen- metaMDBG (15, 1, 1, 1.8, 8); hifiasm-meta (18, 1, 3, 10.7, 125); metaFlye (183, 1, 3, 5, 37). The data to generate this boxplot have been extracted from Supplementary Table S5.