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. 2001 Jan;75(1):362–374. doi: 10.1128/JVI.75.1.362-374.2001

FIG. 5.

FIG. 5

FIG. 5

Subcellular localization of Vpx. vTF7-3 infected HeLa cells were transfected with wild-type and mutant Vpx expression plasmids. Twenty-four hours following transfection, the expressed Vpx proteins were detected by indirect immunofluorescence with an anti-Vpx monoclonal antibody (74) followed by an anti-mouse FITC-conjugated secondary antibody. (a) Localization of Vpx; (b) staining of cytoplasmic (red) and nuclear (blue) compartments with Texas red-phalloidin and DAPI, respectively; (c) superimposition of images shown in panels a and b for each row.