TABLE 1.
Summary of putative BGCs of Chlorogloeopsis ULAP02 using different genome mining tools.
| Identification | Chlorogloeopsis sp. | Chlorogloeopsis fritschii. | |||
|---|---|---|---|---|---|
| Strain | ULAP02 | C42_A2020_084 | PCC 9212 | PCC 6912 | |
| NCBI RefSeq Assembly | GCF_038404745.1 | GCF_015272425.1 | GCF_000317265.1 | GCF_000317285.1 | |
| Type of environment | Isolation source | Copper mine tailings | Non-acidic hot spring microbial mat | Water, thermal spring | Soil |
| Geographical location | Philippines | Chile | Spain | India | |
| Genome Features | Estimated genome size (Mb) | 7.7 | 7.1 | 7.6 | 7.8 |
| GC Content (%) | 41 | 41.5 | 41.5 | 41.5 | |
| Number of contigs | 68 | 241 | 188 | 161 | |
| N50 (kb) | 227.5 | 46.6 | 118 | 123.9 | |
| L50 | 12 | 45 | 19 | 20 | |
| Genes | 6,774 | 6,354 | 6,853 | 6,951 | |
| Protein coding genes | 6,577 | 6,152 | 6,674 | 6,763 | |
| Completeness (%) | 99.64% | 99.27% | 99.70% | 98.97% | |
| Contamination (%) | 3.77% | 1.72% | 2.18% | 2.18% | |
| Sequencing Platform | Illumina NovaSeq | Illumina HiSeq | 454 | 454 | |
| Genome coverage | 100x | 16.7x | 25x | 27x | |
| Assembly level | Scaffold | Contig | Contig | Contig | |
| Assembly method | SPAdes v. 3.15.3 | SPAdes v. v3.13.0 | GS De Novo Assembler v. 2.3 | GS De Novo Assembler v. 2.0.01.14 | |
| Genome Mining Tools | AntiSMASH | ||||
| Terpene | 2 | 2 | 2 | 2 | |
| NRPS | 4 | 5 | 4 | 4 | |
| NRPS-like | 1 | 4 | 4 | 4 | |
| Lanthipeptide class V | 1 | 1 | 1 | 1 | |
| Lasso peptide | 1 | 1 | - | - | |
| hgIE-KS | 3 | 3 | 3 | 2 | |
| Resorcinol | 1 | 1 | 1 | 1 | |
| Indole | 1 | 1 | 1 | 1 | |
| T1PKS | 1 | 4 | 4 | 4 | |
| T3PKS | 1 | 1 | 1 | 1 | |
| Linear azole(in)e-containing peptide (LAP) | 1 | — | — | — | |
| Phosphonate | — | 1 | 1 | 1 | |
| Lanthipeptide class II | — | — | 2 | 2 | |
| NRP-metallophore | — | 1 | 1 | 1 | |
| Mycosporine-like | — | 1 | 1 | 1 | |
| Trans-AT-PKS-like | — | — | 1 | 1 | |
| deepBGC | |||||
| Polyketide | 15 | 18 | 16 | 15 | |
| Saccharide | 9 | 18 | 18 | 13 | |
| NRP | 10 | 6 | 6 | 6 | |
| RiPP | 5 | - | 9 | 11 | |
| Terpene | 2 | 6 | 4 | 4 | |
| Antibacterial | 56 | 99 | 98 | 87 | |
| Cytotoxic | 4 | 5 | 9 | 7 | |
| Inhibitor | 1 | — | 1 | 1 | |
| PRISM | |||||
| NRP | 7 | 5 | 5 | 5 | |
| Prochlorosin | 1 | 2 | 3 | 3 | |
| Lassopeptide | 1 | 1 | — | - | |
| Class II Lantipeptide | — | — | 2 | 2 | |
| Resorcinol | 1 | — | 1 | 1 | |
| Polyketide | 6 | 6 | 5 | 5 | |
| Maleimide indolocarbazole | 1 | 1 | 1 | 1 | |
| Phosphonate | 1 | 1 | 1 | 1 | |
| Genome Mining Tools (cont.) | RiPPMiner | Lassopeptide | Lassopeptide | Lanthipeptide B | Lanthipeptide B |
| NaPDoS2 | |||||
| Ketosynthase (KS) Domain | |||||
| Bacillus subtilis FAS | 1 | 1 | 2 | 2 | |
| Jamaicamide | 2 | 3 | 2 | 2 | |
| Photobacterium PUFA | 2 | 3 | 3 | 3 | |
| Shewanella PUFA | 1 | — | — | — | |
| Nosperin | 2 | — | 1 | 1 | |
| Puwainaphycin | 1 | 1 | 1 | 1 | |
| Macrolactin | 1 | — | — | — | |
| Schizochytrium PUFA | 2 | 1 | 1 | 1 | |
| Eschericoli FAS | 1 | - | — | — | |
| Moritella PUFA | 1 | 2 | 2 | 2 | |
| Cylindrocyclophane | — | 1 | 1 | 1 | |
| Disorazole | — | 1 | 1 | 1 | |
| Hectochlorin | — | 1 | 1 | 1 | |
| Nostopeptolide | — | 2 | — | - | |
| Salinomycin | — | 1 | 1 | 1 | |
| Swinholide | — | 1 | — | — | |
| Difficidin | — | — | 1 | 1 | |
| Condensation (C) Domain | |||||
| Microcystin | 11 | 4 | 2 | 2 | |
| Nostopeptolide | 13 | 8 | 7 | 7 | |
| Tubulysin | 2 | — | — | — | |
| Viomycin | 1 | — | — | — | |
| Nodularin | — | 1 | 1 | 1 | |
| Pyoverdine | — | 1 | 1 | 1 | |
| Anabaenopeptilide | — | — | 1 | 1 | |