Fig. 4.

a Alanylation of wild-type minihelix^Ala (MH) and its mutants at positions 3–70 by the mixture of 0.5 μM AlaRS-α and AlaRS-β. b Alanylation of MH, its mutants at positions 3–70, and the RNA tetraloop substrate shown in Fig. 2b by 5 μM AlaRS-α. c Alanylation of MH, its mutants at positions 2–71, and tRNA^Gly by 5 μM AlaRS-α. d Alanylation of MH and its mutants at position 73 by 5 μM AlaRS-α. The concentration of RNA substrates was 15 μM. Error bars represent the SEM of triplicate experiments