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. 2020 May 7;88(6):501–509. doi: 10.1007/s00239-020-09945-1

Table 1.

Kinetic parameters for aminoacylation of tRNAAla and minihelixAla by N. equitans AlaRS

AlaRS-α + AlaRS-β kcat (s−1) Km (μM) kcat/Km
(μM−1 s−1)
kcat/Km
(relative)
tRNAAla 3.0 ± 0.36 1.6 ± 0.067 1.9 ± 0.31 1
MinihelixAla 1.9 ± 0.46 2.1 ± 0.42 0.9 ± 0.44 0.47
AlaRS-α kcat (s−1) Km (μM) kcat/Km
(μM−1 s−1)
kcat/Km
(relative)
tRNAAla 0.021 ± 0.0021 7.7 ± 0.93 0.0027 ± 0.00059 1
MinihelixAla 0.028 ± 0.00050 23 ± 1.6 0.0012 ± 0.00030 0.44

The data represent the mean of triplicate experiments ± SEM