FIG. 1.

Kinetics of rep-cap amplification upon adenovirus infection. HeRC32 cells were infected with Ad5 at an MOI of 50. Total genomic DNA extracted at 24, 48, and 72 h postinfection was digested with PstI and analyzed on a Southern blot using a rep probe (1.4 kb) obtained by digesting plasmid pspRC with PstI. The position of the expected 1.4-kb rep band is indicated. The standard samples with 1, 10, and 100 rep-cap copies per cell were obtained by adding 36, 360, and 3,600 pg, respectively, of plasmid pspRC to 10 μg of total genomic DNA from noninfected HeLa cells. Lane 1, DNA from adenovirus-infected HeLa cells; lanes 2, 3, and 4, standard rep-cap genome copies; lane 5, DNA from noninfected HeRC32 cells; lanes 6, 7, and 8, DNA extracted from HeRC32 cells 24, 48, and 72 h post-adenovirus infection, respectively.