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. 2024 Sep 12;15:7998. doi: 10.1038/s41467-024-52396-1

Fig. 1. The flow cytometry-based assay.

Fig. 1

a Flow cytometry dot plot outlined monocyte subsets based on CD14 and CD16 expression, displaying surface Aβ detection on monocytes via W0-2 and secondary antibodies in the histograms. b Confocal microscopy displayed Aβ peptides on the surface and cytoplasm of intermediate monocytes, identified by CD14 and CD16 (n = 3). Intracellular Aβ was observed using the 6E10 mAb and captured through Z-stack imaging (n = 2). Nuclei were counter-stained with DAPI. MFI: The mean fluorescence intensity.