Fig. 2. The affinity of monocytes to synthetic Aβ_1-42 peptides.

Human blood samples (n = 6, all CU) were incubated with synthetic Aβ_1-42 peptides to validate the assay’s specificity. a Monocytes showed significant binding compared to the DMSO (circled). Lymphocytes and neutrophils did not show comparable binding. Among monocyte subsets, CD14⁺CD16⁺ and CD14⁻CD16⁺ monocytes exhibited significant Aβ_1-42 binding (circled). CD14⁺CD16⁻ monocytes did not show binding. b, c These findings confirm the specific binding of monocytes to Aβ_1-42 peptides, particularly CD14⁺CD16⁺ and CD14⁻CD16⁺ monocytes. The column dot plots represent mean ± standard deviation. All datasets were normally distributed. Group comparison was determined by one-way ordinary ANOVA, followed by Tukey’s multiple comparisons test (solid zig-zag line).