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. 2024 Sep 12;15:7998. doi: 10.1038/s41467-024-52396-1

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© The Author(s) 2024

Open Access This article is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License, which permits any non-commercial use, sharing, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if you modified the licensed material. You do not have permission under this licence to share adapted material derived from this article or parts of it. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by-nc-nd/4.0/.

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Fig. 7 — PBMCs were sourced from donor C57BL/6 mice and labeled with CFSE (n = 13) or from CX3CR1-eGFP mice without CFSE labeling (n = 5). Both sets of PBMCs were injected into the lateral ventricles of APP/PS1 mice between 15 and 80 weeks of age. Two days later, recipients’ brains, deep cervical lymph nodes (dcLN), and blood were examined using fluorescent flow cytometry. a, d, and g display typical flow cytometry light scatter dot plots of the brain, dcLN, and blood, respectively. The presence of donors’ CFSE⁺ cells in the recipients’ brains confirmed successful delivery (circled in b). Likewise, CFSE⁺ cells in recipients’ dcLN (circled in e) and blood (circled in h, j) validated peripheral migration. No CFSE⁺ cells were found in the recipients’ blood neutrophil gating (k). Notably, the donors’ CX3CR1-eGFP⁺ monocytes were identified as carrying substantial amounts of Aβ in the recipients’ brain (asterisked in c), dcLN (asterisked in f) and blood (asterisked in l). A few CX3CR1-eGFP⁺ monocytes were found in the recipients’ blood lymphocyte gating (i) due to an overlap between lymphocytes and monocytes. This flow cytometry analysis demonstrates the migration and Aβ-carrying capabilities of adoptive PBMCs in different tissues. C: Cells; L: Lymphocytes; M: Monocytes; and N: Neutrophils.