Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2024 Sep 12;15:7921. doi: 10.1038/s41467-024-51978-3

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2024

Open Access This article is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License, which permits any non-commercial use, sharing, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if you modified the licensed material. You do not have permission under this licence to share adapted material derived from this article or parts of it. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by-nc-nd/4.0/.

PMC Copyright notice

Fig. 3 — a, b Heat map and volcano plot showing differentially expressed genes when comparing chronically stimulated (Day 15) to baseline (Day 8) CART19-28ζ cells (DESEQ2 with three biological replicates, padj < 0.05). In the volcano plot, significantly differentially expressed genes (padj < 0.05) are colored red. c Top differentially regulated pathways as determined by QIAGEN IPA of differentially expressed genes (DESEQ2 with three biological replicates, padj < 0.05). d, e Motifs enriched in chronically stimulated as compared with baseline CART19-28ζ cells (MEME/TOMTOM analysis with three biological replicates). f, g Top canonical pathways and upstream regulators as identified by QIAGEN IPA of genes that were both differentially expressed (DESEQ2 with three biological replicates, padj < 0.05) and differentially accessible (DiffBind with DESEQ2 using three biological replicates, padj < 0.05). h Ratio of CD4⁺ to CD8⁺ CART19-28ζ cells in baseline (Day 8) and chronically stimulated (Day 15) cell populations from the in vitro model for exhaustion (Paired two-sided t test, average of two technical replicates for three biological replicates, mean ± SD). i The percent of CD4⁺ CART19-28ζ cells that are Th2 polarized as determined by CCR6^-CCR4⁺CXCR3^- via flow cytometry in baseline (Day 8) and chronically (Day 15) stimulated CART19-28ζ cell populations (Paired two-sided t test, average of two technical replicates for three biological replicates, mean ± SD). j The percent of either CD3⁺, CD4⁺, or CD8⁺ CART19-28ζ cells producing IL-4 as determined by intracellular staining for IL-4 by flow cytometry following four hours of antigen-specific CAR stimulation through co-culturing Day 8 or Day 15 CART19-28ζ cells with JeKo-1 cells at a 1:5 effector-to-target (E:T) cell ratio (Two-way ANOVA, average of two technical replicates for three biological replicates, mean ± SD). Source data for (h–j) are provided in the Source Data file.