Fig. S1.

WNK1 suppresses osteoclast formation in a NLRP3-dependent manner. (A) The mRNA level of WNK1 in transfected macrophages was detected by qRT-PCR. n = 3. (B) The protein levels of WNK1 in transfected macrophages was detected by Western blotting. n = 3. (C) The mRNA level of NLRP3 in transfected macrophages was detected by qRT-PCR. n = 3. (D) The protein levels of NLRP3 in transfected macrophages was detected by Western blotting. n = 3. (E) RANKL-stimulated osteoclastogenesis in transfected macrophages was monitored by TRAP assay with quantitative analysis. The percentage of TRAP⁺ multinucleated cells were counted and calculated. n = 3. (F-H) The mRNA levels of osteoclast-specific molecules in transfected macrophages were detected by qRT-PCR. n = 3. (I) The protein levels of osteoclast-specific molecules in transfected macrophages were detected by Western blotting with quantitative analysis. n = 3. *, P < 0.05; **, P < 0.01; ***, P < 0.001 assessed by Student's t-test (A-D) or One-way ANOVA with Turkey post hoc test (E-I).