Skip to main content
. 2024 Aug 27;48:190–203. doi: 10.1016/j.jot.2024.08.008

Figure 1.

Figure 1

METTL14, HOXA5 and WNK1 are downregulated in OVX mice with pyroptosis. (A) The histological changes of femur were detected by H&E staining. Scale bar: 100 μm. n = 6 per group. (B) The serum levels of IL-1β and IL-18 were measured by ELISA assay. n = 6 per group. (C) The expression of IL-1β and IL-18 in the femur were detected by qRT-PCR. n = 6 per group. (D) The immunoreactivities of NLRP3, METTL14, HOXA5 and WNK1 in the femur were examined by IHC analysis. Scale bar: 100 μm. n = 6 per group. (E&F) The mRNA levels of METTL14 and HOXA5 in the femur were detected by qRT-PCR. n = 6 per group. (G) The protein levels of METTL14, HOXA5, WNK1 and pyroptosis-related proteins in the femur were detected by Western blotting with quantitative analysis. n = 6 per group. *, P < 0.05; **, P < 0.01; ***, P < 0.001 as assessed by Student's t-test.