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. 2024 Aug 27;48:190–203. doi: 10.1016/j.jot.2024.08.008

Figure 2.

Figure 2

Overexpression of METTL14 or HOXA5 suppresses macrophage-osteoclast differentiation. (A, C) The mRNA levels of METTL14 and HOXA5 in transfected macrophages were detected by qRT-PCR. n = 3. (B, D) The protein levels of METTL14 and HOXA5 in transfected macrophages were detected by Western blotting. n = 3. (E) RANKL-stimulated osteoclastogenesis in transfected macrophages was monitored by TRAP assay with quantitative analysis. The percentage of TRAP+ multinucleated cells were counted and calculated. n = 3. (F) The secretion of CTX-1 was assessed by ELISA assay. n = 3. (G–I) The mRNA levels of osteoclast-specific molecules in transfected macrophages were detected by qRT-PCR. n = 3. (J) The protein levels of osteoclast-specific molecules in transfected macrophages were detected by Western blotting with quantitative analysis. n = 3. *, P < 0.05; **, P < 0.01; ***, P < 0.001 as assessed by Student's t-test (A–D) or One-way ANOVA with Turkey post hoc test (E–J).