Figure 3.

METTL14 or HOXA5 overexpression upregulates WNK1 expression and suppresses pyroptosis in macrophages. (A) LDH release in transfected macrophages was monitored using commercial kit. n = 3. (B&C) The mRNA levels of IL-1β and IL-18 in transfected macrophages were detected by qRT-PCR. n = 3. (D&E) The secreted IL-1β and IL-18 levels in the cell culture supernatant were measured by ELISA assay. n = 3. (F) The populations of caspase-1⁺/PI⁺ cells were analyzed by flow cytometry with quantitative analysis. n = 3. (G) The protein levels of WNK1 and pyroptosis-related proteins in transfected macrophages were detected by Western blotting with quantitative analysis. n = 3. *, P < 0.05; **, P < 0.01; ***, P < 0.001 as assessed by One-way ANOVA with Turkey post hoc test.