Figure 4.

METTL14-mediated m⁶A modification stabilizes HOXA5 mRNA and increases its expression. (A, C) The mRNA level of METTL14 or HOXA5 in transfected macrophages was detected by qRT-PCR. n = 3. (B, D) The protein level of METTL14 or HOXA5 in transfected macrophages was detected by Western blotting with quantitative analysis. n = 3. (E) The m⁶A modification of HOXA5 in transfected macrophages was evaluated by MeRIP. n = 3. (F) HOXA5 mRNA stability in transfected macrophages was assessed by RNA stability assay. n = 3. *, P < 0.05; **, P < 0.01; ***, P < 0.001 as assessed by Student's t-test.