Figure 6.

Knockdown of WNK1 reverses METTL14-or HOXA5-suppressed pyroptosis in macrophages. (A) The mRNA level of WNK1 in transfected macrophages was detected by qRT-PCR. n = 3. (B) The protein level of WNK1 in transfected macrophages was detected by Western blotting with quantitative analysis. n = 3. (C) LDH release in transfected macrophages was monitored using commercial kit. n = 3. (D&E) The mRNA levels of IL-1β and IL-18 in transfected macrophages were detected by qRT-PCR. n = 3. (F&G) The secreted IL-1β and IL-18 levels in the cell culture supernatant were measured by ELISA assay. n = 3. (H) The populations of caspase-1⁺/PI⁺ cells were analyzed by flow cytometry with quantitative analysis. n = 3. (I) The protein levels of WNK1 and pyroptosis-related proteins in transfected macrophages were detected by Western blotting with quantitative analysis. n = 3. *, P < 0.05; **, P < 0.01; ***, P < 0.001 as assessed by Student's t-test (A&B) or One-way ANOVA with Turkey post hoc test (C–I).