Figure 7.

Silencing of WNK1 counteracts METTL14-or HOXA5-suppressed macrophage-osteoclast differentiation. (A) RANKL-stimulated osteoclastogenesis in transfected macrophages was monitored by TRAP assay with quantitative analysis. The percentage of TRAP⁺ multinucleated cells were counted and calculated. n = 3. (B–D) The mRNA levels of osteoclast-specific molecules in transfected macrophages were detected by qRT-PCR. n = 3. (E) The protein levels of osteoclast-specific molecules in transfected macrophages were detected by Western blotting with quantitative analysis. n = 3. *, P < 0.05; **, P < 0.01; ***, P < 0.001 as assessed by One-way ANOVA with Turkey post hoc test.