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. 2024 Aug 27;48:190–203. doi: 10.1016/j.jot.2024.08.008

Figure 8.

Figure 8

Overexpression of METTL14 or HOXA5 alleviates osteoporosis via suppressing WNK1-dependent NLRP3 signaling in vivo. (A) The histological changes of femur were detected by H&E staining. Scale bar: 100 μm. n = 6 per group. (B–D) BV/TV, Tb.N and Tb.Th were measured by micro-CT. n = 6 per group. (E) The serum level of CTX-1 was assessed by ELISA assay. n = 6 per group. (F&G) The serum levels of IL-1β and IL-18 were measured by ELISA assay. n = 6 per group. (H&I) The expression of IL-1β and IL-18 in the femur were detected by qRT-PCR. n = 6 per group. (J) The immunoreactivity of NLRP3 in the femur was examined by IHC analysis. Scale bar: 100 μm. n = 6 per group. (K) The protein levels of METTL14, HOXA5, WNK1 and pyroptosis-related proteins in the femur were detected by Western blotting with quantitative analysis. n = 6 per group. (L) The protein levels of METTL14 and HOXA5 in BMDMs were detected by Western blotting with quantitative analysis. n = 6 per group. *, P < 0.05; **, P < 0.01; ***, P < 0.001 as assessed by One-way ANOVA with Turkey post hoc test.