FIGURE 2.

Small RNAseq of DLD‐1 cells and sEVs treated with 5‐FU. Small RNAseq was performed on RNA (<200 nt) isolated from DLD‐1 cells and sEVs treated or not with 5‐FU. (a) Small RNA read totals normalized to reads per million (RPM) from the indicated RNA subclasses after RNAseq on cellular and sEV RNA isolated in the presence or absence of 5‐FU. RNA subclasses include long noncoding RNA‐derived RNAs (lncDRs), miRNAs, miscellaneous small RNAs, rRNA‐derived RNAs (rDRs), spliceosomal snRNA‐derived RNAs (snDRs), snoRNA‐derived RNAs (snoDRs), tRNA‐derived RNAs (tDRs) and Y RNA‐derived RNAs (yDRs). (b) Distinct clustering of miRNA expression patterns across triplicate RNAseq samples in cells and sEVs after treatment with 5‐FU.