FIG. 4.

BJAB/73 cells express ORF73-specific mRNA and the LANA protein. (A) Northern blot analysis of RNA extracted from BJAB cells transfected with pCEP4/73 (lane 1) or pCEP4 (lane 2) and from BCBL-1 cells (lane 3). BJAB cells were maintained in selection medium containing hygromycin B for 4 weeks prior to the analysis. Each lane contains 10 μg of total RNA. A SmaI/BamHI fragment containing the N-terminal domain of LANA was used to prepare a radioactively labeled probe. We have previously shown that BCBL-1 cells express two transcripts from this region (indicated by arrows) (24). (B) Immunofluorescence patterns of BJAB cells transfected with pCEP4/73 or pCEP4 and of BCBL-1 cells. Cells were reacted with a 1:400 dilution of a LANA-specific polyclonal rabbit antiserum.