Figure 6.

Urethane treatment of A/J mice may induce both small and non-small lung cancer. (A) Representative H&E staining of formaldehyde-fixed paraffin-embedded sections from A/J mice lungs treated with PLGA-PEI-si-NT, showing two different morphologies as visualized in images at three different magnifications. (B,C) Representative lung sections of PLGA-PEI-si-NT-treated mice, IHC stained using specific antibodies against KI-67 (B) and synaptophysin (C), known to be highly expressed in SCLC. The squares show the areas from the high and low IHC-stained tumors that were magnified 25-fold in the images shown on the right. (D) Quantitative analysis of the indicated proteins’ staining intensities. Results reflect the mean ± SEM (n = 3), **** p ≤ 0.0001.