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. 2001 Jan;75(1):480–489. doi: 10.1128/JVI.75.1.480-489.2001

FIG. 6.

FIG. 6

FIG. 6

CARex-Fc-mediated increase of Ad transgene expression in hematopoietic cells. (A) Jurkat, HL-60, THP-1, and primary AML cells were transduced with AdCMV-eGFP using an MOI of 10 in the absence (red) or presence (green) of 1 μg of CARex-Fc protein. Cells alone are shown as solid blue histograms. (B) MOI and CARex-Fc dose effects on the transduction efficiency of THP-1 cells. Results are given as means of duplicate determinations of eGFP mean values. The results of one of three comparable experiments are shown. (C) Inhibition of CARex-Fc-mediated transgene expression by preincubation of THP-1 cells with 2 μg of CTLA4-Ig for 30 min on ice. Experimental settings were comparable to those in panel B when using AdCMV-eGFP at an MOI of 10 and various amounts of CARex-Fc protein. (D) Inhibition of transgene expression by preincubation of THP-1 cells with 1 μg of an monoclonal mouse anti-human CD64 antibody.