Table 5. Minimum bactericidal concentrations (MBC) and selectivity indices (SI) for 25% TFA-16-PLL₁₀ and 16-PLL₁₀ for a range of clinical isolates recovered from wounds.

Bacteria name	ID no.	Site	16-PLL10	16-PLL10	TFA-16-PLL10	TFA-16-PLL10
			MBCμM	SIIC50/SIMHC*–	MBCμM	SIIC50/SIMHC*–
Acinetobacter baumannii	23AD06	BAL	1.25	1.5/2	2.5	1.8/4
Acinetobacter lwoffii	22AD05	Foot Ulcer	1.25	1.5/2	1.25	0.9/2
E. faecium (VRE)	23AD04	BKA Wound	5	5.9/8	2.5	1.8/4
Enterobacter cloacae complex	23AD01	Foot Ulcer	>40	>47/>64	>40	>28.6/>64
Klebsiella oxytoca	23AD11	Foot Ulcer	40	47/64	>40	>28.6/>64
Pseudomonas aeruginosa	22AD01	Ankle Ulcer	≤0.156	<0.18/<0.25	≤0.156	<0.11/<0.25
S. aureus (MRSA)	23AD02	Necrotic Toe	5	5.9/8	2.5	1.8/4
S. aureus (MSSA)	23AD10	Foot Ulcer	10	11.7/16	10	7.2/16
Streptococcus dysgalactiae	23AD09	Foot Ulcer	2.5	2.9/4	2.5	1.8/4
All isolates†	–	–	4.3‡	5.0/6.8	4.0‡	2.8/6.3

MBC - , VRE - Vancomycin resistant enterococci, MRSA - Methicillin resistant , MSSA - Methicillin susceptible , MHC – Minimum haemolytic concentration that caused haemolysis, *SI_IC50 - selectivity index based on IC₅₀, SI_MHC - selectivity index based on MHC. Selectivity index calculated by dividing MBC by IC₅₀ value (0.85 µM - 16-PLL₁₀, 1.4 µM TFA-16-PLL₁₀) and MHC_5% (0.625 µM). Selectivity index calculated by dividing the geometric mean of MBC by IC value or MHC value. Geometric mean of MBC (where MBC and, values of and used in calculation of geometric mean.

†Selectivity index calculated by dividing the geometric mean of MBC by IC₅₀ value or MHC value.

‡Geometric mean of MBC (where MBC >40 µM and ≤0.156 µM, values of 80 µM and 0.156 µM used in calculation of geometric mean).

MBCminimum bactericidal concentrationMHCminimum haemolytic concentration that caused 5% haemolysisMRSAmethicillin resistant S. aureusMSSAmethicillin susceptible S. aureusVREvancomycin resistant enterococci