Table 1.

Primer sequences used for a quantitative real-time polymerase chain reaction.

Gene	Primer Sequence		Product Size	Accession Number
PAK4	forward	5′-GGACATCAAGAGCGACTCGAT-3′	113	NM_001014831.3
	reverse	5′-CGACCAGCGACTTCCTTCG-3′
PD-L1	forward	5′-GGACAAGCAGTGACCATCAAG-3′	235	NM_001267706.2
	reverse	5′-CCCAGAATTACCAAGTGAGTCCT-3′
FOXO3	forward	5′-CGGACAAACGGCTCACTCT-3′	150	NM_001455
	reverse	5′-GGACCCGCATGAATCGACTAT-3′
CCND1 (Cyclin D1)	forward	5′-GAGGAAGAGGAGGAGGAGGA-3′	236	NM_053056.2
	reverse	5′-GAGATGGAAGGGGGAAAGAG-3′
P27	forward	5′-TCTACTGCGTGGCTTGTCAG-3′	240	AB001740.1
	reverse	5′-CTGTATTTGGAGGCACAGCA-3′
BAX	forward	5′-CCCGAGAGGTCTTTTTCCGAG-3′	155	NM_138763
	reverse	5′-CCAGCCCATGATGGTTCTGAT-3′
BCL2	forward	5′-GGTGGGGTCATGTGTGTGG-3′	89	NM_000657
	reverse	5′-CGGTTCAGGTACTCAGTCATCC-3′
SNAL1 (Snail)	forward	5′-ACCCCACATCCTTCTCACTG-3′	217	NM_005985.3
	reverse	5′-TACAAAAACCCACGCAGACA-3′
TGF- β1	forward	5′-CCCACAACGAAATCTATGACAA-3′	246	NM_000660.7
	reverse	5′-AAGATAACCACTCTGGCGAGTG-3′
MMP2	forward	5′-GATACCCCTTTGACGGTAAGGA-3′	112	NM_004530
	reverse	5′-CCTTCTCCCAAGGTCCATAGC-3′
MMP9	forward	5′-TGTACCGCTATGGTTACACTCG-3′	97	NM_004994
	reverse	5′-GGCAGGGACAGTTGCTTCT-3′
GAPDH	forward	5′-AACAGCGACACCCACTCCTC-3′	258	NM_001256799.1
	reverse	5′-GGAGGGGAGATTCAGTGTGGT-3′

Web link to accession numbers: https://www.ncbi.nlm.nih.gov/gene (accessed on 10 January 2024).