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D4Z4 methylation in human sperm samples, determined using DBS. Scatter plot showing D4Z4 methylation levels in 188 sperm samples (blue dots) from males with an age range from 26 to 54 years. The horizontal line indicates the 2.5% methylation threshold. The vast majority (135 of 188) of samples have methylation values between 0% and 2.5%.
