Table 1.
Gene | Primer Sequence (5′-3′) | Product Size (bp) | Annealing Temperature (°C) |
---|---|---|---|
PKM2 | F: GGCACCCACGAGTATCAT | 205 | 60 |
R: CATTGTCCAGCGTCACTTT | |||
HK1 | F: CCTCTTGGCTTCACATTC | 231 | 60 |
R: TTCACAGTTTGGGTCTTCAT | |||
HIF1A | F: TTGACAAGGCATCCATTA | 157 | 60 |
R: TCCTCAGAAAGCACCATA | |||
LDHA | F: TGGGCATCCATCCTCTGA | 168 | 60 |
R: CCTGCTTGTGAACCTCCT | |||
GLUT1 | F: TGTTTGGCTTGGACTTGAT | 171 | 60 |
R: TCTTGAGGACGCTCTTGG | |||
PFKP | F: GCCACAACAAACCTATAACA | 241 | 60 |
R: ATCAAAGGCAGACGAACA | |||
β-actin | F: GAACCCCAAAGCCAACAGAGAG | 142 | 60 |
R: ATCACCAGAGTCCATCACAATACCA |