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. 2024 Aug 28;14(17):2500. doi: 10.3390/ani14172500

Table 1.

Primers used for qRT-PCR.

Gene Primer Sequence (5′-3′) Product Size (bp) Annealing Temperature (°C)
PKM2 F: GGCACCCACGAGTATCAT 205 60
R: CATTGTCCAGCGTCACTTT
HK1 F: CCTCTTGGCTTCACATTC 231 60
R: TTCACAGTTTGGGTCTTCAT
HIF1A F: TTGACAAGGCATCCATTA 157 60
R: TCCTCAGAAAGCACCATA
LDHA F: TGGGCATCCATCCTCTGA 168 60
R: CCTGCTTGTGAACCTCCT
GLUT1 F: TGTTTGGCTTGGACTTGAT 171 60
R: TCTTGAGGACGCTCTTGG
PFKP F: GCCACAACAAACCTATAACA 241 60
R: ATCAAAGGCAGACGAACA
β-actin F: GAACCCCAAAGCCAACAGAGAG 142 60
R: ATCACCAGAGTCCATCACAATACCA