Figure 4.

Identifying mPR-Specific pathways Using combined Non-mPR Progesterone Action Filters. The objective of this experiment was to identify signaling pathways specifically associated with the membrane progesterone receptor (mPR) by excluding the PRG actions that are not mPR-specific across the distinct CCM1 genotypes defined in the previous step (Supplementary Figure S3A). This approach aimed to generate mPR-specific signaling pathways in response to PRG actions via CCM1, as represented below. (A) To visualize the ORA results of core-enriched DEPs from GO and KEGG pathway enrichments, an integrative dot plot was used. The plot displayed data from three CCM1 genotypes that passed the filter for non-mPR-specific PRG actions. Triplicate analysis was conducted, and statistical significance was determined using a Student’s t-test with a p-value cut-off of less than 0.05. The integrative dot plot highlighted the enriched pathways associated with mPR-specific PRG action, indicated by red-framed, blue-framed, and black pathways. (B) Similarly, an integrative dot plot was employed to visualize the ORA signaling pathways for mPR-specific PRG actions of enriched RNAseq data from GO and KEGG pathway enrichments. These data, which underwent triplicate analysis, passed through the filter for non-mPR-specific PRG actions among the two CCM1 genotypes used in the RNAseq portion of this study. The statistical significance of the results was determined using a Student’s t-test, with a p-value cut-off of less than 0.05. (C) Finally, a summarized dot plot was created to visualize the combined ORA signaling pathways for mPR-specific PRG actions, highlighting the core-enriched DEPs and DEGs from both proteomic and RNAseq data.