Figure 7.

Effect of 19,20-DHDP on TNT formation and proteins in endothelial cell–pericyte junctions. (a) Heatmap showing the 50 pericyte–endothelial cell junctions most affected by 19,20-DHDP (10 µM, 16 h). Mitochondrial proteins are highlighted in red, PDGFR-β signaling in blue, and trafficking-related proteins in green; n = three independent cell batches. (b) Volcano plot showing endothelial cell–pericyte junctional proteins in co-cultures of endothelial cells and pericytes, comparing 19,20-DHDP with non-treated fractions (19,20-DHDP/Solvent). (c) Pathway enrichment analysis (NCI-Nature) of proteins significantly decreased in the junctional space after exposure to 19,20-DHDP. (d) Representative Western blot showing the effect of PDGFBB (1 ng/mL, for up to 240 min) on the phosphorylation of the PDGF receptor, Akt, and Erk1/2 in pericytes in the absence and presence of 19,20-DHDP; n = four independent cell batches.