Figure 4.

Drug-mediated inhibition of cell proliferation and PARylation and effects on survival and apoptosis protein markers. Cells were seeded in T25 flasks overnight and exposed to individual drugs or three-drug combinations for 3 days, harvested, and analyzed for cell proliferation by MTT assay (A) and Western blotting (B). For the double- and triple-drug combinations, the drug concentrations were the same as indicated in the single-drug concentrations. Model-adjusted means are shown with 95% confidence intervals, and solid points indicate a significant synergistic difference from all of the component drugs (Supplemental Table S3). Each cell line of each drug was modeled independently. Number of replicates = 3; Pano/P: panobinostat; SAHA/S: vorinostat; TLZ/T: talazoparib; Ola/O: olaparib; DAC/D: decitabine.