Figure 4.

F26 inhibits ACAT activity in the forebrain, cerebellum/brain stem, adrenal gland, and liver after a single IP administration of NP F26 to WT mice at (A) 6 h, (B) 24 h, (C) 48 h time points, respectively. WT mice were injected with either PBS, vehicle, NP F12511 (46 mg/kg), or NP F26 (51 mg/kg). Relative ACAT activity was measured by using a mixed micelle assay. Bars represent mean ± SD. Each data point represents a triplicate for each mouse (n = 2–3/group). p-value determined using two-way ANOVA followed by Tukey’s multiple comparisons test. # showed statistical significance compared to PBS, and * showed statistical significance compared to the vehicle. #,* p < 0.05, ##,** p < 0.01, ###,*** p < 0.001, ####,**** p < 0.0001.